Associate Professor,Department of Conservative Dentistry & Endodontics,Vinayaka mission’s Sankarachariyar Dental College & Research Foundation ( Deemed to be University),Salem,Tamilnadu State, India. Pin Code Number-636004 ;
Corresponding Author: dr.vanita99@gmail.com
Professor and Head, Department of Pediatric and preventive dentistry, Chettinad dental college and Research Institute, Chennai, India, postcode-603103;
Corresponding Author: ayaswathi@gmail.com;
Professor, Department of Pediatric and Preventive Dentistry, Chettinad Dental College and Research Institute, Chennai, India, 603103;
Corresponding Author: dr.eaga_ars@yahoo.com
Department of Pedodontics and Preventive Dentistry, Chettinad Dental College & Research Institute, Rajiv Gandhi Salai, Kelambakkam, Kancheepuram District, Tamil Nadu – 603 103, India
On human dental enamel, the remineralisation efficiency of a subsurface enamel defect was evaluated using powder extracts from three marine skeletal species: Crassostrea madrasensis (oyster shell), Perna viridis (mussel shell), and Penaeus monodon (shrimp shell).Methods: The study included 60 freshly extracted human third molar teeth that were sectioned at the cement-enamel junction (CEJ). Later, each tooth coronal region was separated into two halves of enamel blocks, yielding 120 blocks that were exposed to the demineralization technique and categorised as follows: Subsurface demineralization + oyster shell powder extract form Group I. Group II: demineralization of the subsurface + mussel shell application Subsurface demineralization + shrimp shell powder extract (Group III). Group IV: subsurface demineralization plus clinpro application, followed by thirty days in artificial saliva. The intact enamel samples served as a positive control, whereas demineralized enamel samples served as a negative control. Using X-ray fluorescence spectroscopy, micro hardness testing, and energy dispersive x-ray spectroscopy,samples were evaluated for remineralisationThe samples were evaluated using X-ray fluorescence spectroscopy, energy dispersive x-ray spectroscopy and micro hardness testing for atomic analysis & surface microhardness. One-way ANOVA and the Tukey – Kramer multiple comparison tests were used for statistical analysis.
[1] Featherstone JDB. Caries prevention and reversal based on the caries balance. Pediatr Dent. 2006;28(2):128-32. discussion 192-8.
[2] Walsh LJ. Contemporary technologies for remineralization therapies: A review. Int Dent SA. 2009;11(6):06-16
[3] . Featherstone JDB, Behrman JM, Bell JE. Effect of whole saliva components on enamel demineralisation in vitro. Crit Rev Oral Biol Med. 1993;4(3-4):357-62.
[4] Walsh LJ. Contemporary technologies for remineralization therapies: A review. Int Dent SA. 2009;11(6):06-16
[5] Walsh LJ. Contemporary technologies for remineralization therapies: A review. Int Dent SA. 2009;11(6):06-16.
[6] Elkassas D, Arafa A. Remineralising efficacy of different calcium-phosphate and fluoride based delivery vehicles on artificial caries like enamel lesions. J Dent. 2014;42(4):466-74.
[7] Green DW, Lai WF, Jung HS. Evolving marine biomimetics for regenerative dentistry. Mar Drugs. 2014;12(5):2877-912. doi: 10.3390/md12052877.
[8] Macha I, Ozyegin L, Chou J, Samur R, Oktar F, Nissan B. An alternative synthesis method for di calcium phosphate (Monetite) powders from mediterranean mussel (Mytilusgalloprovincialis) shells. Journal of The Australian Ceramic Society. 2013;49(2):122-28.
[9] .Islam K N, Bakar ZBA, Noordin MM, Hussain MZB, Rahman NSBA, Ali E. Characterisation of calcium carbonate and its polymorphs from cockle shells (Anadara granosa). Powder Technology. 2011;213(1-3):188-91.
[10].Ivancakova R, Hogan MM, Harless JD, Wefel JS. Effect of fluoridated milk on progression of root surface lesions in vitro under pH cycling conditions. Caries Res. 2003;37:166-71. doi: 10.1371/journal.pone.0104327.
[11]Ivancakova R, Hogan MM, Harless JD, Wefel JS. Effect of fluoridated milk on progression of root surface lesions in vitro under pH cycling conditions. Caries Res. 2003;37:166-71. doi: 10.1371/journal.pone.0104327. .
[12]. Lata S, Varghese NO, Varughese JM. Re [12] Lata S, Varghese NO, Varughese JM. Remineralisation potential of fluoride and amorphous calcium phosphate-case in phospho peptide on enamel lesions: An in vitro comparative evaluation. J Conserv Dent. 2010;13(1):42-46.
[13]Tabakaeva OV, Tabakaev AV, Piekoszewski W. Nutritional composition and total collagen content of two commercially important edible bivalve molluscs from the Sea of Japan coast. J Food Sci Technol. 2018;55(12):4877-86. doi: 10.1007/ s13197-018-3422-5.
[14] Macha IJ, Ben-Nissan B. Marine skeletons: Towards hard tissue repair and regeneration. Mar Drugs. 2018;16(7):225. doi: 10.3390/md16070225.
[15]Hoque ME, Shehryar M, Islam KMN. Processing and characterization of Cockle Shell Calcium Carbonate (CaCO3 ) bioceramic for potential application in bone tissue engineering. J Material Sci Eng. 2013;2(4):01-05. doi: 10.4172/2169- 0022.1000132.
[16] Awang-Hazmi AJ, Zuki ABZ, Noordin MM, Jalila A, Norimah Y. Mineral composition of the cockle (Anadara granosa) shells of west coast of peninsular Malaysia and it’s potential as biomaterial for use in bone repair. Journal of Animal and Veterinary Advances. 2007;6(5):591-94. Kamba AS, Ismail M, Ibrahim TAT, Zakaria ZAB. Synthesis and characterisation of calcium carbonate aragonite nanocrystals from cockle shell powder 14 (Anadaragranosa). Journal of Nanomaterials. 2013;2013:398357. https://doi. org/10.1155/2013/398357