Evaluation of Remineralising potential and Microhardness of three different marine skeletal species – Crassostrea madrasensis , Perna viridis and Penaeus monodon on human teeth enamel — An invitro study”

Dr.Vanita D.Revankar

Associate Professor,Department of Conservative Dentistry & Endodontics,Vinayaka mission’s Sankarachariyar Dental College & Research Foundation ( Deemed to be University),Salem,Tamilnadu State, India. Pin Code Number-636004 ;

Corresponding Author: dr.vanita99@gmail.com

Daya Srinivasan

Professor and Head, Department of Pediatric and preventive dentistry, Chettinad dental college and Research Institute, Chennai, India, postcode-603103;

Corresponding Author: ayaswathi@gmail.com;

Senthil Eagappan

Professor, Department of Pediatric and Preventive Dentistry, Chettinad Dental College and Research Institute, Chennai, India, 603103;

Corresponding Author: dr.eaga_ars@yahoo.com

R.A Sowmiyasree

Department of Pedodontics and Preventive Dentistry, Chettinad Dental College & Research Institute, Rajiv Gandhi Salai, Kelambakkam, Kancheepuram District, Tamil Nadu – 603 103, India

Abstract :

On human dental enamel, the remineralisation efficiency of a subsurface enamel defect was evaluated using powder extracts from three marine skeletal species: Crassostrea madrasensis (oyster shell), Perna viridis (mussel shell), and Penaeus monodon (shrimp shell).Methods: The study included 60 freshly extracted human third molar teeth that were sectioned at the cement-enamel junction (CEJ). Later, each tooth coronal region was separated into two halves of enamel blocks, yielding 120 blocks that were exposed to the demineralization technique and categorised as follows: Subsurface demineralization + oyster shell powder extract form Group I. Group II: demineralization of the subsurface + mussel shell application Subsurface demineralization + shrimp shell powder extract (Group III). Group IV: subsurface demineralization plus clinpro application, followed by thirty days in artificial saliva. The intact enamel samples served as a positive control, whereas demineralized enamel samples served as a negative control. Using X-ray fluorescence spectroscopy, micro hardness testing, and energy dispersive x-ray spectroscopy,samples were evaluated for remineralisationThe samples were evaluated using X-ray fluorescence spectroscopy, energy dispersive x-ray spectroscopy and micro hardness testing for atomic analysis & surface microhardness. One-way ANOVA and the Tukey – Kramer multiple comparison tests were used for statistical analysis.

Keywords:
  • Calcium,
  • Minimal invasive dentistry
  • Phosphate
  • X-ray fluorescence spectroscopy
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© The Author(s), under exclusive license to Technoarete Publishers 2022
  • ISBN - 978-93-92104-02-2
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  • Total No. of. Pages - 150
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https://dx.doi.org/10.36647/ETOHSD/2022.01.B1.Ch023