Abstract : Conventional agriculture's widespread use of chemical fertilisers and pesticides has led to serious health and environmental problems, including the rise of infections and pests that are resistant to these treatments. With their dual advantages as biofertilizers and biocontrol agents, Plant Growth Promoting Rhizobacteria (PGPR) provides a sustainable substitute. This study explores how PGPR promotes plant development through pathogen control, phytohormone synthesis, and nutrient solubilisation. The economic feasibility and use of PGPR, especially under abiotic stress situations, are also investigated. By increasing nutrient absorption and generating growth-promoting compounds, PGPR enhances plant development directly. It also inhibits phytopathogens indirectly through processes like siderophore synthesis and lytic enzyme release. Despite its promise, strain specificity, formulation stability, and regulatory obstacles are obstacles to PGPR's commercialization. The study emphasizes the necessity of continuing studies to improve our comprehension of plant-microbe interactions and create more reliable PGPR formulations. Reducing reliance on synthetic agrochemicals and incorporating PGPR into conventional agriculture techniques would require addressing these issues. Adopting PGPR successfully may result in more sustainable farming methods that support healthier crops and ecosystems. Additionally, our current work, which is based on this evaluation of the literature, clarifies important technical issues that may arise both before and during the interpretation of DNA chromatograms in Sanger sequencing and provides solutions. This study emphasises how important it is to recognise and address these technological constraints throughout the chromatogram analysis. Acknowledging these issues can help enhance the quality of downstream studies for Sanger sequencing data, which provide significant improvements in accuracy, dependability, and capacity to supply critical genetic information in clinical analysis.

Keyword : Chromatogram, Electropherograms, Phytopathogens, Plant Growth Promoting Rhizobacteria (PGPR), Sanger sequencing, Siderophore synthesis and Lytic enzyme.